2003 - 2013

November 17-23, 2013

Ljubljana, Slovenia

Electroporation based Technologies and Treatments

International SCIENTIFIC WORKSHOP and POSTGRADUATE COURSE

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Key lectures and content of the course:

Electrical properties of cells and tissues in electric field

  • Resting and induced transmembrane voltage
  • Dielectric properties of biological tissues
  • Temperature and conductivity changes in tissues
  • Physical chemistry of membrane electroporation

  • Electrochemical membrane thermodynamics
  • Molecular dynamics simulations
  • Electroporation in vitro and in vivo - protocols

  • Electroporation of cells in vitro
  • Electroporation of cells in vivo
  • Development of devices and electrodes

  • Devices for in vitro and in vivo experimental work
  • Electrodes for in vitro and in vivo
  • Electroporation of cells in tissue - models of electroporation in vivo and their experimental validations
  • Electrochemotherapy of tumors

  • Basic mechanisms of electrochemotherapy
  • Preclinical studies
  • Clinical use of electrochemotherapy
  • Current developments and future perspectives
  • Application of electroporation in gene transfection

  • Gene transfection in vitro
  • Gene transfection in vivo
  • Clinical use in human medicine
  • Electroporation in transdermal drug delivery

  • Electroporation for gene delivery in the skin
  • Electroporation for DNA vaccine
  • Additional topics:

  • Electroporation based research and resource efficient process development for healthy foods, Dietrich Knorr
  • Microbial inactivation by Pulsed Electric Fields; Fundamentals and Applications, Javier Raso
  • DNA Vaccination protocols assisted by electrotransfer, Emanuela Signori
  • Gaining access to intracellular compartments by electroporation, James Weaver
  • Laboratory and practical work

  • Gene electrotransfer with different pulse parameters
  • Monitoring cell membrane electropermeabilization with ratiometric fluorescence dye FURA-2AM
  • Electropermeabilization detection of attached cells with propidium iodide
  • Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS
  • Electroporation of bacteria
  • Electroporation of planar lipid bilayers
  • Cell electrofusion
  • Electrochemotherapy treatment planning: optimization of voltage and electrode position
  • Numerical modeling of coupled physical phenomena
  • Molecular dynamics simulations of membrane electroporation
  • Electrochemotherapy - interactive learning